Author, Subject, and Keyword

Sterilization- biologics, proteins and peptides

“Sterilization of epidermal growth factor with supercritical carbon dioxide and peracetic acid; analysis of changes at the amino acid and protein level”

Cell Removal and Tissue Cleaning

Decellularised Tissues Obtained by a CO2-philic Detergent and Supercritical CO2.

Eur Cell Mater 36:81-95 (2018)

In Vivo Immunogenicity of Bovine Bone Removed by a Novel Decellularization Protocol Based on Supercritical Carbon Dioxide

Artif Cells Nanomed Biotechnol 46:334-344 (2018)

Decellularised Tissues Obtained by a CO2-philic Detergent and Supercritical CO2.

Eur Cell Mater 36:81-95 (2018)

A Novel Supercritical CO2-based Decellularization Method for Maintaining Scaffold Hydration and Mechanical Properties

J Supercritical Fluids 131:72-81 (2018)

Supercritical Carbon Dioxide Decellularised Pericardium: Mechanical and Structural Characterisation for Applications in Cardio-Thoracic Surgery

J Mech Behav Biomed Mater 77:400-407 (2018)

Technology Review

Supercritical CO2 Technology: The Next Standard Sterilization Technique? Mater Sci Eng C Mater Biol Appl 99:520-540 (2019)

Detergent-Free Decellularization

Cell Removal with Supercritical Carbon Dioxide for Acellular Artificial Tissue
J Chem Technol Biotechnol 83:943–949 (2008)

Preparation of Acellular Scaffold for Corneal Tissue Engineering by Supercritical Carbon Dioxide Extraction Technology.
Acta Biomater 58:238-243 (2017)

Supercritical Carbon Dioxide-Assisted Decellularization of Aorta and Cornea
Tissue Eng Part C Methods 9:540-547 (2017)

Supercritical Carbon Dioxide Extracted Extracellular Matrix Material From Adipose Tissue
Mater Sci Eng C Mater Biol Appl 75:349-358 (2017)

Decellularized Heart ECM Hydrogel Using Supercritical Carbon Dioxide for Improved Angiogenesis
Acta Biomater 67:270-281 (2018)

Supercritical Carbon Dioxide Decellularization of Plant Material to Generate 3D Biocompatible Scaffolds
Sci Rep 11, 3643 (2021)


Fast decellularization process using supercritical carbon dioxide for trabecular bone
The Journal of Supercritical Fluids,  Vol 172 (2021)

Whole Lung

Sterilization of Lung Matrices by Supercritical Carbon Dioxide Tissue Eng Part C Methods 22:260-269 (2016)

Bone Products

Does implantation site influence bone ingrowth into 3D-printed porous implants? The Spine Journal 19 (2019)

The use of demineralised bone fibres (DBF) in conjunction with supercritical carbon dioxide (SCCO2) treated allograft in anterior lumbar interbody fusion (ALIF) Journal of Spine Surgery  Vol 5, No 4 (December 2019)

Supercritical Carbon Dioxide- Sterilized Bone Allograft in the Treatment of Tunnel Defects in 2-Stage Revision Anterior Cruciate Ligament Reconstruction- A Histological Evaluation The Journal of Arthroscopic and Related Surgery Vol 34, Issue 3, p 706-713 (March 2018)

Meniscal Allograft Sterilisation: effect on Biomechanical and Histological Properties
Cell Tissue Bank 16:467-475 (2015)

The Effect of Supercritical Carbon Dioxide Sterilization on the Anisotropy of Bovine Cortical Bone
Cell Tissue Bank 16:109-121 (2015)

The Effect of Sterilization Methods on Osteoconductivity of Allograft Bone on a Critical-Sized Bilateral Tibia Defect Model in Rabbits
Biomaterials 34:8185-8194 (2013)

Russell NA, Rives A, Bertollo N, Pelletier MH, Walsh WR
The Effect of Sterilization on the Dynamic Mechanical Properties of Paired Rabbit Cortical Bone
J Biomech 46:1670-1675 (2013)

Russell NA, Rives A, Pelletier MH, Bruce WJ, Walsh WR
The Effect of Sterilization on the Mechanical Properties of Intact Rabbit Humeri in Three-Point Bending, Four-Point Bending and Torsion
Cell Tissue Bank 14:231-242 (2013)

Dental Membranes (pericardium)

In Vivo Evaluation of Resorbable Supercritical CO2-Treated Collagen Membranes for Class III Furcation-Guided Tissue Regeneration
J Biomed Mater Res B Appl Biomater [Epub ahead of print]


Sun Y, Lovric V, Wang T, Oliver R, Walsh WR
Effects of SCCO2, Gamma Irradiation, and Sodium Dodecyl Sulfate Treatments on the Initial Properties of Tendon Allografts
Int. J. Mol. Sci. 2020, 21(5), 1565;

Effects of Supercritical Fluid COand 25 kGy Gamma Irradiation on the Initial Mechanical Properties and Histological Appearance of Tendon Allograft
Cell Tissue Bank 19:603-612 (2018)

Nichols A, Burns DC, Christopher R
Studies on the Sterilization of Human Bone and Tendon Musculoskeletal Allograft Tissue Using Supercritical Carbon Dioxide

J Orthopaedics 6:e9 (2009)


Biomaterial Characterization of Off-the-shelf Decellularized Porcine Pericardial Tissue for Use in Prosthetic Valvular Applications
J Tissue Eng Regen Med  12:1608-1620 (2018)

Scaffolds Impregnation

Combined sterilization and fabrication of drug-loaded scaffolds using supercritical CO2 technology
Intl J of Pharmaceutics Volume 612, (25 January 2022)

Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration
Intl J of Pharmaceutics Volume 605, (10 August 2021)

Drug Loading of Polymer Implants by Supercritical CO2 Assisted Impregnation: A Review
J Control Release 209:248-259 (2015)

Amniotic Membranes

Development of a Sterile Amniotic Membrane Tissue Graft Using Supercritical Carbon Dioxide
Tissue Eng Part C Methods 7:649-659 (2015)

Sterilization – Microbial Inactivation

Evaluation of supercritical CO2 sterilization efficacy for sanitizing personal protective equipment from the coronavirus SARS-CoV-2
Science of The Total Environment Volume 780,(August 2021)

Mechanism of Bacillus subtilis Spore Inactivation by and Resistance to Supercritical CO2 plus Peracetic Acid
J Appl Microbiol 120:57-69 (2016)

Improved Sterilization of Sensitive Biomaterials with Supercritical Carbon Dioxide at Low Temperature
PLoS One 10:e0129205 (2015)

Inactivation of Bacterial Spores and Viruses in Biological Material Using Supercritical Carbon Dioxide with Sterilant
J Biomed Mater Res Pt B:  Appl Biomater 91B:572-578 (2009)

Effective Terminal Sterilization using Supercritical Carbon Dioxide
Biotechnol123:504-515 (2006)

Alginate/Collagen Scaffolds

Effect of scCO2 on the decontamination of PECs-based cryogels: A comparison with H2O steam and H2O2 nebulization methods

International Journal of Pharmaceutics, Volume 646,2023, 123451, ISSN 0378-5173

Sterilization – Applications

Inactivation of Legionella in Aqueous Media by High-Pressure Carbon Dioxide
The Journal of Supercritical Fluids 180 (2022) 105431

Sterilization of Biological Weapons in Technical Clothing and Sensitive Material by High-Pressure CO2 and Water
Ind. Eng. Chem. Res 2019, 57, 4680-46870

Recellularization of a Novel Off-the-shelf Valve Following Xenogenic Implantation Into the Right Ventricular Outflow Tract
PLoS One 12:e0181614 (2017)

Supercritical Carbon Dioxide-Based Sterilization of Decellularized Heart Valves
JACC Basic Transl Sci 2:71-84 (2017)

Absorbable sutures

Novel Supercritical Carbon Dioxide Sterilization of Absorbable Sutures and new Amino Acid-Based Biomaterials as Suture Coatings
NovaScience Publications.  Biodegradable Polymers. Volume 1: Advancement in Biodegradation Study and Applications, Chapter 14 pp.381-406 (2015)


Synergistic Effect of Supercritical Carbon Dioxide and Peracetic Acid on Microbial Inactivation in Shredded Mozzarella-type Cheese and its Storage Stability at Ambient Temperature
Food Control 70:174-182 (2016)

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