Improved Sterilization of Sensitive Biomaterials with Supercritical Carbon Dioxide at Low Temperature

Anne Bernhardt, Markus Wehrl, Birgit Paul, Thomas Hochmuth, Matthias Schumacher, Kathleen Schütz, Michael Gelinsky

Centre for Translational Bone, Joint and Soft Tissue Research, Medizinische Fakultät, Technische Universität Dresden, Dresden, Germany, 2 wfk—Cleaning Technology Institute e.V., Krefeld, Germany

PLOS ONE | DOI:10.1371/journal.pone.0129205 June 12, 2015


Development of a Sterile Amniotic Membrane Tissue Graft Using Supercritical Carbon Dioxide

Wehmeyer Jennifer L., Natesan Shanmugasundaram, and Christy Robert J. 

Tissue Engineering Part C: Methods. July 2015, 21(7): 649-659. doi:10.1089/ten.tec.2014.0304.


The effect of supercritical carbon dioxide sterilization on the anisotropy of bovine cortical bone.

Russell Nicholas, Rives Alain, Pelletier Matthew H, Wang Tian, and Walsh William. Cell Tissue Bank. April 2014, doi:10.1007/s10561-014-9447-6


Meniscal allograft sterilization: effect on biomechanical and histological properties.

Bui David, Lovric Vedran, Oliver Rema, Bertollo Nicky, Broe David, and Walsh William 

Cell Tissue Bank. December 2014, doi:10.1007/s10561-014-9492-3


Sterilisation treatments of corticancellous allograft in a rabbit model.

Ganeshalingam R, Oliver R, Musgrove T, and Yu Y. 

Orthopaedic Proceedings. 2012, 94-B(Supp XXIII):124.


The effect of sterilization on the mechanical properties of intact rabbit humeri in three-point bending, four-point bending and torsion.

Russell Nicholas, Rives Alain, Pelletier Matthew, Bruce Warwick, and Walsh William.
Cell Tissue Bank. May 2012, doi:10.1007/s10561-012-9318-0


Supercritical carbon dioxide technology improves food safety


Studies on the sterilization of human bone and tendon musculoskeletal allograft tissue using supercritical carbon dioxide.

Nichols Anastasia. 

Journal of Orthopaedics. 2009, 6(2)e9


Inactivation of Bacterial Spores and Viruses in Biological Material Using Supercritical Carbon Dioxide With Sterilant

Qing-Qing Qiu, Patrick Leamy, Jennie Brittingham, Jason Pomerleau, Nimesh Kabaria, Jerome Connor Department of Research, LifeCell Corporation, Branchburg, New Jersey 08876

Received 10 December 2008; revised 25 March 2009; accepted 15 April 2009

Published online 6 July 2009 in Wiley InterScience ( DOI: 10.1002/jbm.b.31431


Effective terminal sterilization using supercritical carbon dioxide.

White Angela, Burns David, Christensen Tim. 

Journal of Biotechnology. December 2005, doi:10.1016/j.jbiotec.2005.12.033